Product
Glysite™ Scout Glycan Screening Kits

Glysite™ Scout Glycan Screening Kits

Glysite™ Scout Glycan Screening Kit, Immunofluorescence 649 (GSK-1000)

Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 (GSK-2000)

Glysite™ Scout Glycan Screening Kit, Immunofluorescence 488 (GSK-3000)

Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, Gal, and LacNAc. In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, streptavidin DyLight™, and VECTASHIELD Vibrance® Antifade Mounting Media with DAPI. Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs.​ Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Supplier: Vectorlabs

 

product specifications:

Enjoy convenience

With our fully integrated kits, everything you need is right at your
fingertips…just add your tissue.

Get results faster

Glycobiology may be complex but getting started doesn’t need to be. We’ve
done the heavy lifting with a curated selection of lectins and an optimized protocol so you can focus on answering your questions

Trust your tools

Backed by 45+ years of experience, you can rely on Vector Laboratories to
support you every step of the way. With over 25,000 glycobiology publications, scientists around the world trust our products to deliver publication-quality results

Have confidence in your results

Leverage our manufacturing excellence to get consistent, reliable results time and time again. Lectin-binding characteristics are also independently assessed by the National Center for Functional Glycomics (NCFG).

Description

 

B-ECL
B – GNL
B-PHA-L

 

 

 

 

 

 

 

 

 

 

 

 

 

 

FFPE human colon carcinoma stained with Glysite Scout Glycan Screening Kit, Immunofluorescence 594 (GSK-2000, red), with colocalization of the pan-cytokeratin antibody AE1/AE3 (green). The tissue was treated with Antigen Unmasking Solution, Citrate-Based (H-3300) and Vector TrueVIEW Autofluorescence Quenching Kit (SP-8400). Nuclear detail was visualized by DAPI staining (blue). Slides were imaged at 20x. Fluorescent green staining (DyLight™ 488) illuminates the location of multiple high and low
molecular weight cytokeratin proteins, stained with AE1/AE3. This staining is juxtaposed with the fluorescent red staining (DyLight 594) of lectins (B-ECL, B-GNL, and B-PHA-L) to demonstrate glycan distribution in the tumor microenvironment.

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