NZYTech Monkeypox virus Real-time PCR Kit, RUO includes all reagents required to identify the presence of monkeypox virus DNA in a variety of relevant samples. DNA isolated and purified from different sample sources is amplified in a single reaction using two highly specific primers/probe sets. The probes specifically anneal to two regions of the monkeypox virus genome, namely B7R and CrmB genes. An additional primers/probe set acts as an endogenous internal control to detect nucleic acids of β-actin.
For research use only;
Triplex target assay (Monkeypox virus B7R and CrmB genes and human β-actin gene);
Positive control to validate assay included;
Internal control to confirm extraction / inhibition included
This test is intended for the in vitro qualitative detection of monkeypox virus genome. A positive result indicates the presence of Monkeypox virus DNA. This kit is intended for use by laboratory trained personnel, specifically instructed in real-time PCR techniques.