TriMethyl-Histone H3-K27 Rabbit pAb
Western blot analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (A2363) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s
Kindly refer to the above description.